The iCARE Malaria P.f/P.v Rapid Test Kit is designed for the qualitative detection of Plasmodium falciparum (P.f) and Plasmodium vivax (P.v) specific antigens in human whole blood. This easy-to-use test offers a rapid way to distinguish between these two types of malaria infections. Here’s a step-by-step guide to using the kit effectively.
Preparation and Precautions
- Ensure the kit is for in vitro diagnostic use only and is not consumed.
- The kit is single-use; do not attempt to reuse it.
- Check the expiration date before use and do not use if the pouch is damaged.
- Handle all specimens as potentially infectious.
- Dispose of the kit and materials properly after use.
- Store the kit at 2~30°C and keep it away from sunlight, moisture, and heat.
Kit Contents
Each kit includes:
- Test cards with a desiccant in a foil pouch.
- A plastic sample dropper (5μl).
- Sample diluent.
- A safety lancet.
- An alcohol swab.
- A package insert with detailed instructions.
Step wise Test Procedure to Use the iCARE Malaria P.f/P.v Home Use Rapid Test Kit
- Room Temperature Adjustment: Before starting, bring the test card, sample diluent, alcohol swab, safety lancet, and plastic dropper to room temperature.
- Opening the Kit: Carefully open the sealed pouch and take out the test card.
- Preparing the Test Site:
- Clean the fingertip with the alcohol swab provided in the kit.
- Use the safety lancet to prick the fingertip to obtain a drop of blood.
- Collecting the Blood Sample:
- Use the plastic dropper to collect the blood from the fingertip. Ensure you collect the correct volume as indicated.
- Applying the Blood Sample:
- Apply the collected blood to the sample well on the test card.
- Adding the Diluent:
- Add the sample diluent to the test card as directed in the package insert. This will facilitate the flow of the sample across the detection area.
Reading the Results
- Wait for 10 minutes before reading the result. The test should not be read after 20 minutes as the results may not be accurate.
- Positive Result: The appearance of two or three lines on the test card, including a control line, indicates a positive result for either P.falciparum or P.vivax malaria.
- Negative Result: A single line in the control region indicates a negative result, meaning no P.falciparum or P.vivax antigens were detected.
- Invalid Result: If no lines appear, or if the control line does not appear, the test is considered invalid. The test should be repeated with a new kit.
Quality Control
- An internal procedural control is included in the test device. However, it is recommended to use external positive and negative controls as good laboratory practice to confirm the assay procedure and verify proper test performance.
Conclusion
The Malaria Test Kits provides a convenient and quick method for detecting malaria infections caused by P.falciparum and P.vivax. By following these steps closely, users can effectively conduct the test at home or in field settings. Remember, any positive result should be followed up with further testing by a healthcare professional for confirmation and appropriate treatment.